Passive Systemic Anaphylaxis (PDF)
- Sensitization:
- Inject 20µg IgE anti-DNP in 100µl NaCl, i.p. (27G 1cc)
- Challenge (24hr later):
- take baseline temperature (N ~38-39°) & mark tails
- inject 1mg of DNP-HSA in 100µl NaCl, i.p.
- read temperature at 10', 20', 30', 40', 50', 60', 90' & 120' (180' Meffi)
